Cooperative Catalysis

Cooperative catalysis occurs when the synergic catalytic effect of at least two different entities acts together, increasing the rate of a chemical reaction beyond what is possible when either of the two entities is used independently. The idea of “Cooperative Catalysis” has inspired synthetic chemists to create artificial dual activation catalysts. Such a Cooperative Catalytic pathway is often used in enzymatic catalysis. Enzymes are continue to be a source of inspiration for (designing and) developing new catalytic reactions that are high in efficiency & selectivity and minimal waste.

Urease is a dinuclear metalloenzyme which catalyzes the hydrolysis of urea into carbon-di-oxide and ammonia. The dimeric nickel center of this enzyme is (the active site) responsible for cooperative catalysis. Urea co-ordinates with one Ni-center, thus activate the electrophile (act as Lewis Acid), whereas water coordinates with the second Ni. It is thus acidified and can be deprotonated by histidine to generate hydroxide as a nucleophile (generate nucleophile), which is, now in close to the electrophile, and, can attack in an intramolecular fashion.

A similar cooperative catalytic activity with Bronsted and Lewis acidities can be generated by simultaneous incorporation of multiple elements in the silica framework is quite interesting and holds promises of unprecedented catalytic performances.

We recently prepared a similar natural mimic, a bimetallic nano-porous catalytic system, which would be able to perform cooperative catalysis for the selective synthesis of ortho-prenylated phenols and 2,2-dimethyl chroman, starting from phenol and allylic alcohol. Prenylated phenols are widely distributed in nature and are known to be an important structural unit of pharmaceutical compounds. Similarly, 2,2-dimethylchroman derivatives also exhibit broad range of interesting physiological properties, we are able to synthesis the important structural motif by using the new cooperative catalytic systems.

The amount of aluminum present in the framework dictates the acidity of the catalyst, and by fine-tuning the aluminum content, we can develop the catalyst with the desired catalytic property. Catalyst developed in such a manner was found to be highly active and selective. The products obtained were good and satisfactory. Additionally, the synergistic effect of the bimetals (Cu and Al) in the nanoporous catalysts controls the selectivity of the final products.

Nobel Advice

Are you a Nobel aspirant, young researcher, or want to be part of good science? Here is an advice from Prof. Avram Hershko ( Winner of 2004 Nobel Prize -for the discovery of ubiquitin-mediated protein degradation)

Here is a lesson from his life in science that he presented at the Lindau Nobel Meeting 2013."

• It is very important to have good mentors- you cant learn how to do good science just from reading the literature.
• Find an important subject that is not yet interesting to others the big guys will get there before you! Do not go with the mainstream. 
• Accidental observations may be the most important ones. Grab your Luck. 
• Use whatever experimental approach is needed for your objective. It may not necessarily be the most fashionable (“state-of-the-art”) technology. Of course, biochemistry will always be needed. 
• Science should be a curious driven adventure. You should have a lot of excitement and fun. 
• Never leave bench work, and shall continue to get a lot of excitement and fun.

Inspired by Nobel

It is a great honor, not to mention my good fortune as well, to be nominated and sponsored by Department of Science and Technology, India, as one of the 23 young researchers who will be attending the 63rd Lindau Nobel Laureate Meeting as part of the Indian delegation.

With much anticipation, I await to listen to the eminent speakers at the Lindau stellar, who need no elaborate introduction. The work of most of the scholars have formed a basis for my own work as well as for thousands of other scientists and students around the world. We ceaselessly draw inspiration from their work. I have long admired these scholars, but to listen to them in person would be an awe-inspiring experience, which I am sure would vouch for it.

“It is the quest for knowledge that drives the scientific community.” Armed with this belief, I look forward to meet and interact with personalities of scientific excellence at close quarters. The informal setting at the Lindau Meeting, I hope, will allow us to discuss our work in a relaxed manner. I believe this meeting will be a great platform to learn directly from the masters and to meet peers from across the globe.

Read More at the Lindau blogs website

Click Chemistry for Pyrrole Synthesis

The Pyrrole heterocycle is an important chemical motif, found widely in pharmaceuticals, natural products, agrochemicals, and advanced materials. The introduction of new methods or further the work on technical improvements in order to overcome the limitations (such as low efficiency and selectivity) found in pyrrole synthesis is still a pressing experimental challenge. 

The concept of “Click Chemistry” is gaining rapidly due to its high efficiency, selectivity, and yield under mild reaction conditions with a wide variety of readily available starting materials. The copper-catalyzed azide-alkyne cycloaddition (CuAAC) has emerged as the premier example of click chemistry and plays a significant role in organic synthesis. 

Prof. Aiwen Lei and coworkers, Wuhan University, Hubei, have developed a silver catalyst “click reaction” for the synthesis of pyrrole, by cycloaddition. This system benefits from readily-available starting materials, low catalyst loading (0.1 eq), short reaction times (2 h), and excellent chemo-selectivity. Moreover it works for both internal and alkyl-substituted terminal alkynes in the presence of many functional groups. The extremely mild conditions used make this reaction synthetically attractive. 

This mechanism involves the formation of silver–acetylide complex and silver–isocyanide complex. Subsequently, the cyclo-addition between complexes would afford the key intermediate complex to be followed by protonation and tautomerization of the intermediate complex to form the desired product. 

The catalytic synthesis protocol tolerates many functional groups, including methylthio, methylsulfonyl, and ethynyl groups. Moreover, alkyl-substituted terminal alkynes were also found to be suitable reaction partners. Interestingly, both Cu(II) and Cu(I) turned out to be ineffective.

Useful chemicals and fuel from carbon dioxide

Carbon dioxide is an abundant, non-toxic, inexpensive, and renewable source of carbon. This makes CO2 the most coveted compound by Green Chemistry enthusiasts. Industries are always on the lookout for ways to enable the effective use of to act as synthetic building blocks for producing fuels like Methane, Di-methyl-ether, and Methanol fine-chemicals. Furthermore, CO2 conversion could also help reduce atmospheric CO2 levels, popularly known as “Green House Gas”, and thus, protect the climate.

Nature has been highly successful in using CO2 as synthetic building blocks in photosynthesis. For decades, scientists have been trying to understand this phenomenon at a molecular level. Such studies have proved useful in developing biomimetic catalysts for CO2 conversion. Chlorophyll (Porphyrin molecules) in green plants convert incident sunlight and atmospheric CO2 into sugars (energy). So, this makes them a promising target for testing activation catalysts for CO2 adsorption. Effective CO2 adsorption using man-made catalysts is indeed our end-goal. Much research is being conducted in this area to further the economic viability of the processes that utilize CO2. Several companies are pursuing the idea/concept of thermochemical and electrochemical conversion of CO2 into chemical feedstock or polymers. Research and development are currently focused on increasing the catalyst life and bringing down the temperature of conversion.

Future research must emphasize the rational design of highly active catalysts to satisfy the economic development of CO2 conversion. However, the development of such efficient catalysts requires a complete understanding of CO2 and CO2-catalyst interactions.

In order to develop such a catalyst, the following points should be considered,

1. CO2 has a strong affinity towards nucleophiles and electron-donating reagents due to its carbonyl-carbon's electron deficiency; if the designed catalysts has nitrogen or base-functionality (basic), it will have an increased affinity towards CO2 (e.g., Porphyrin, Grignard reagents).
2. With low-valent metals and alkene, CO2 undergoes “oxidative cycloaddition.”
3. New CO2 soluble catalysts may increase efficiency.
4. Homogeneous catalysis in compressed CO2 may increase selectivity.
5. The catalyst in supercritical CO2 may also increase stability. It is essential to use CO2, based on the unique physical properties as that of the supercritical fluid, either as a solvent, or as an anti-solvent, or reactant, or a combination of all.
6. Photoelectrochemistry, the study of using solar energy to split CO2, is an emerging method for clean production of chemicals. It is also essential to develop catalysts (Semiconducting materials) for the electrochemical conversion of CO2.
7. The use of high-energy starting materials may ease the catalyst role.
8. The catalyst will be more efficient if it has both CO2 adsorption and activation functionality. E.g., designer MOF that contains Lewis-base sitewill donate electron to CO2, in contrast to the Lewis-acids sites in traditional MOFs for adsorbing CO2.
9. A computational tool such as Density Function Theory (DFT) may help improve the catalytic activity or find a new catalyst.
10. Chemical reactions can also benefit from using CO2 as a mild oxidant or as a selective source of O2 atoms because dissociation of CO2 on a catalyst-surface could produce active O2 species.

The trend towards converting CO2 to valuable chemicals and fuels will probably intensify in the near future. This could, in turn, lead to effective management to tackle climate change and the energy crisis.

As Whitesides emphasizes, managing CO2 and conversion into valuable chemicals and energy will be the reinvention of chemistry, and it is also a chemistry/ molecular solution to the critical problem facing society. He says

 “Some of the most interesting problems in science, and many of the most important facing society, need chemistry for their solution. Examples include: understanding life as a network of chemical reactions; interpreting the molecular basis of disease; global stewardship; the production, storage, and conservation of energy and water; and the management of carbon dioxide."

Issues pertaining to CO2 are truly global and a major opportunity to develop sustainable energy options and environmental preservation. So, the use of CO2 to synthesize useful chemicals & fuels will mark a new field in chemistry. It is important to establish university-industry-collaboration to search for new-reactions & new-catalysts in this field.

Hope for Drug to Reduce Old-Age-Related Muscle Wasting

Guest Post – Lily Bryant

The results of a study carried out by researchers at the Center of Regenerative Medicine at Massachusetts General Hospital in Boston, USA, and the Department of Craniofacial Development and Stem Cell Biology at King’s College London, England, indicate that a cure for muscle weakening caused by old age might be on the horizon. Muscle wasting is an inevitable symptom of growing older. It can be slowed down by regularly exercising but there is no known way of reversing it at the moment. This looks set to change though, as the researchers have treated old mice with a drug that has significantly improved the ability of their aged muscle tissue to self-repair. 

Fibroblast Growth Factor 2 

Strenuous activity, such as doing press-ups or lifting weights, results in a small level of damage to the muscles. Stem cells are triggered to repair them by dividing and developing into new muscle fibers. This results in big arms and rippling torsos. However, weakening of the muscles due to old age means that bulging biceps can soon become puny and weak. This occurs because as people grow older, their muscle loses its ability to regenerate. A study conducted by the University of Potsdam in Germany indicates that people lose just under a third of their muscle strength between the ages of fifty and seventy. The researchers at Massachusetts General Hospital and King’s College London deduced that this is due to the fact that the number of dormant stem cells in muscles decreases with age. They concluded that in mice, this was caused by excessively high levels of a protein that stimulates cells to divide, known as fibroblast growth factor 2 (FGF2). 

Preventing Muscle Stem Cell Decline 

In aging muscle, fibroblast growth factor 2 was found to be continuously awakening dormant stem cells for no reason. The stem cell supply was observed to deplete over time, meaning that an insufficient number were available when they were really needed. As a result of this, muscle regeneration ability was impaired. The researchers discovered that using a drug called SU5402 that inhibits fibroblast growth factor 2 can prevent muscle stem cell decline in aging mice. Treating the elderly rodents with this drug increased their ability to repair muscle tissue. 

Reducing the Impact of Old Age 

Dr Albert Basson, who is a senior researcher at King’s College London, has stated that the findings mean that treatments that could make old muscles young again could one day be developed, thus reducing the impact of old age. He says that if such a treatment came into existence, people would be able to live more independent, mobile lives as they grew older. Senior author Dr Andrew Brack of Massachusetts General Hospital says that just as it is important for athletes to schedule recovery into the time that is taken for training, stem cells require time in which to recuperate but elderly cells recuperate less often. Scientists are yet to figure out why it is that levels of fibroblast growth factor 2 increase causing excessive stem cell activation as people get older. Research team member Kieran Jones of King’s College London says that the next step is to conduct an analysis of old muscle in humans in order to see whether or not the same mechanism is responsible for the depletion of stem cells in human muscle fibers leading to wasting and loss of mass. 

The Implications of the Research 

Once it has been confirmed that stem cells in human muscle fibers are negatively affected by fibroblast growth factor 2, work can start on introducing a drug to the market that will stop the process from taking place and consequently reduce the extent to which elderly people are immobilized and prevented from doing what they want to do by the effects of age-related muscle weakening. The average life expectancy in India has increased by over four and a half years since 1998. Now that people are living longer than they used to live for, a drug that reduces the amount to which becoming elderly negatively impacts upon the quality people’s lives would be extremely beneficial. It would mean that individuals who have reached the later stages of their lives could enjoy the time that they have left without having their independence limited by their muscles becoming increasingly weak and feeble.

About Lily Bryant

Lily Bryant is a writer working with one of only two licensed online pharmacies in the US. She is strongly interested in promoting and creating content aimed at relevant readers as part of her role in ethical healthcare business. She believes that it is important that we play a strong role in leading society towards a healthier lifestyle through the promotion of exercise and healthy diet rather than an early adoption of drug treatment.

Artificial Photosynthesis using graphite Carbon Nitride

Fine chemicals and hydrogen production from water, carbon dioxide, and solar energy are ideal future chemical and energy sources independent of fossil reserves. The development of new functional molecular materials (catalyst) for the application in fine chemical and clean energy production using water and solar energy is fascinating and quite challenging because the catalyst must be sufficiently efficient, stable, inexpensive, and capable of harvesting light. 

Polymeric graphite carbon nitride (C₃N₄) materials are commonly available simple semiconductor photocatalysts. It is being non-volatile up to 600⁰C even in the air with no detectable solubility or reactivity in conventional solvents, including water, alcohols, DMF, THF, diethyl ether, and toluene. Carbon nitride is considered to be extremely stable and basic in nature. It can be used as the multifunctional heterogeneous catalyst for fine chemical and pharmaceutical synthesis as well as a good organic semiconductor due to its right bandgap (2.7 eV corresponding to an optical wavelength of 460 nm). 

Prof. Markus Antonietti and his team at Max Planck Institute of Colloids and Interfaces in Germany, have successfully split CO2 or photochemically turn water into hydrogen and oxygen using graphite carbon nitride. However, only four micromoles of hydrogen per hour were produced out of the researcher's reaction vessel (quantum efficiency of the Pt modified CN is approximately 0.1% with irradiation of 420-460 nm). This opens the door to artificial photosynthesis and produces chemicals and energy from greenhouse gas /solar energy. It will contribute to the prevention of global climate change. 

Harmless Viruses Might be a Potential Treatment for Acne

Guest Post – Lily Bryant

According to a report published in the Indian Journal of Dermatology, Venereology and Leprology, there are thought to be between two million and three million acne sufferers in India. Acne can lead to low self-esteem and image problems and is the scourge of teenage boys all over the country. Fortunately towards the end of 2012, researchers at the University of California, Los Angeles, and the University of Pittsburgh in the United States discovered that phages living on our skin possess the ability to kill propionibacterium acnes, which is the virus that causes it. This could be good news for those whose skin is tarnished by this condition and mean that an end is in sight.

There has been a rapid increase in the promotion of drugs aimed at enhancing individuals’ physical appearances in recent years. The weight loss industry has grown at a yearly rate of over twenty-five percent, with more people than ever purchasing diet pills and fat burners, the hair loss treatment industry has been growing by one percent per year and the acne treatment industry has also seen significant growth. People’s daily lives are affected in a major way by how they look so a cure for acne could be both highly profitable for the pharmaceutical company that is responsible for it and highly beneficial to sufferers.

Immune Resistance Unlikely to Develop

In order to arrive at their discovery, the researchers at the two universities analysed phages and deduced that they make endolysin, which breaks down propionibacterium acnes before killing it. They also discovered that the phages shared eighty-five percent of their DNA. This is unusual for viruses and means that when used as a treatment, it would be unlikely that immune resistance would develop.

Advantages Over Other Forms of Treatment

Unlike antibiotics, which kill many different types of bacteria including ones that live in the gut and can have positive effects, phages are programmed to only target specific bacteria. According to director of the University of California, Los Angeles, Clinic for Acne, Rosacea and Aesthetics Dr Jenny Kim, who was one of the researchers, many acne strains are now resistant to antibiotics such as tetracycline, as they are so widely used. Drugs like Accutane are still effective but can have risky side effects, which limits their use. The researchers at the two universities have stated that phages could offer a tailored therapy that has less adverse side effects. Therefore phages could be the new form of acne medication that those inflicted with the condition have been looking for.

Development of New Drugs and Treatments 

Study co-author Graham Hatfull, who is a biotechnology professor at the University of Pittsburgh, has stated that there are two ways in which this research could be used with regards to the development of new drugs and treatments. He says that phages could either be used directly as therapy for acne or phage-based components could be utilized. Professor Hatfull also says that the work that the University of California, Los Angeles, and the University of Pittsburgh have carried out has provided the world with useful information about phages and paved the way for the thinking up of potential applications for them. He points out that whilst acne is a condition that a significant percentage of the population is likely to suffer from at some point in their lives, there are still currently few effective methods for curing it. Hatfull says that harnessing a virus that naturally preys upon the bacteria that causes spots looks to be a promising means of reducing both the physical and the mental scars that acne can inflict upon individuals.

Implications of the Research

It appears that being forced to endure pimples and spots could soon bee a thing of the past. The results of a study published in the Journal of Cosmetic Dermatology in 2010 demonstrate that acne can severely negatively impact upon the self-esteem of sufferers and increase the risk of them developing psychological disorders. It can make them two to three times more likely to become clinically depressed. Therefore it is high time that there was a means of treating it and phages could provide the answer. Now it is just a matter of deciding what the best way of using them to gain the optimum results in acne reduction is.

About Lily Bryant

Lily Bryant is a writer working with one of only two licensed online pharmacies in the US. She is strongly interested in promoting and creating content aimed at relevant readers as part of her role in ethical healthcare business. She believes that it is important that we play a strong role in leading society towards a healthier lifestyle through the promotion of exercise and healthy diet rather than an early adoption of drug treatment.

Modulators of Protein–Protein Interactions

Protein-protein interactions (PPI) play a crucial role in most biological processes. This nature of PPI has put forward itself as a prospective candidate for therapeutic intervention. Traditional small molecule target classes such as Enzymes, GPCRs, Kinases, etc. have a deep pocket (often used to bind an endogenous substrate), where small molecules tend to bind. On the other hand, PPIs appear to be too large and featureless for small molecules to bind against. Hence, due to this lack of well-defined binding pockets, they were considered unsuitable/ extremely hard for targeting small molecules. 

Attempts at generating small molecule modulators of PPIs have been largely unsuccessful by adopting existing chemical techniques. This leads us to believe that we need to identify novel chemical space that can leverage the flat and expansive surfaces of PPI, which would in turn provide an effective binding for small molecules. However, pharmaceutical companies are rather unwilling to add compounds containing multiple rings, multiple stereocenters that are highly complex, into their corporate collection as it does not align with their immediate short-term business goals.

Heterocyclic compounds (aromatic, largely flat and hydrophobic)

+

Natural products (rich in sp2 bonds)

=

Natural Product Inspired   (New Chemical toolbox)

Dr. Prabhat Arya is developing a new chemical toolbox enriched with both Heterocyclic Compounds and Natural Products to tackle such issues from Dr. Reddy’s Institute of Life Sciences. This approach could create a large 3D surface area, numerous binding interactions, rich stereochemical diversity, which would, in turn, solve the poor cell permeability of natural products, not to mention the added advantage of overcrowded IP Space.

The field of small-molecule-PP interactions appears to be highly promising, and in the near future, we can hope to see several strategies and techniques that will pave the way towards discovering novel agents in this regard.