Recently Aronov from Vertex pharmaceuticals proposed (JMC, 2008, 51,1214) a general ‘rule of thumb’ termed the 2-0 rule of kinase likeness to discriminating kinase compound from general compound .
For kinase activity the molecule should have
1) One or more hetero aromatic nitrogen’s
2) One or more hetero aromatic NH group
3) It contain one or more aniline
4) And it contain one or more nitriles
78 % of the kinase compound pass the 2-0 rule.